flowAI report

Quality control analysis

Summary

The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 78.66 %
Number of high quality events: 218904

Flow rate check

14.97 % anomalies detected in the flow rate check.

The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.

Signals acquisition check

77.87 % anomalies detected in the signal aquisition check.

The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.

The FCS file was divided in 2052 bins, hence each bin contains 500 event(s). The stable region is located between the bins 1165 and 1618.

More info on the changepoints detected

Changepoints detected in channels with shifts in signal acquisition:

##                 1    2
## 488 525/50-A  518 1626
## 488 715/30-A  775     
## 405 450/50-A 1912     
## 405 515/20-A 1623     
## 405 605/40-A  788     
## 405 670/30-A  502     
## 355 740/35-A 1829     
## 355 820/60-A 1811     
## 640 730/35-A 1786     
## 561 585/15-A  938     
## 561 610/20-A  286 1165
## 561 780/60-A  503     
## 405 585/15-A 1618

Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.

No changepoints were detected in the channels: 405 710/40-A, 405 780/60-A, 355 379/28-A, 355 515/30-A, 355 560/40-A, 640 670/30-A, 640 780/60-A.

Dynamic range check

1.85 % anomalies detected in dynamic range check.

The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.

More info on the anomalies detected in the dynamic range check

For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:

##              lower_range upper_range
## 488 525/50-A        1270        3679
## 488 715/30-A         258          42
## 405 450/50-A         225          15
## 405 515/20-A         164        1711
## 405 605/40-A         163         108
## 405 670/30-A         183          20
## 405 710/40-A         220          14
## 405 780/60-A        1075           4
## 355 379/28-A         366          62
## 355 515/30-A         126          21
## 355 560/40-A         196         129
## 355 740/35-A         264          11
## 355 820/60-A        1305           6
## 640 670/30-A         476          13
## 640 730/35-A         770          12
## 640 780/60-A        1007         894
## 561 585/15-A         373       13431
## 561 610/20-A         214        4408
## 561 780/60-A         548         464
## 405 585/15-A         209        1438
## total_SUM           9412       26482
## total_UNIQUE        2493       16520